The radio waves had weakened the bonds that held together the elements that made up the water, and ignited the hydrogen. The results left scientists excited by the possibility of separating hydrogen -- the most abundant element in the universe -- from salt water to use as a fuel.
Rustum Roy, a Penn State University chemist and water science expert, called it the most remarkable discovery in water science in the last century. His team is working on the saltwater project at Penn State, using Kanzius' machine.
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The saltwater discovery pleased Kanzius, but the cancer project took precedence.
Four years after he came up with his idea, researchers continued experiments and killed human cancer cells in petri dishes using nanoparticles and his machine. They recently killed 100% of cancer cells grown in the livers of rabbits, using Kanzius' method.
Curley said the treatment is the most promising he has ever seen because it has the potential to kill cancer -- without invasive treatment or surgery -- that doctors currently have no way of detecting. The next step for scientists is to perfect a method of binding nanoparticles with antibodies that, when introduced into the bloodstream, will attach only to cancer cells while avoiding normal cells. He said the treatment could work on any kind of cancer, and he estimates clinical trials are three to four years away.
"Possible?" Curley said. "Yes. Not simple."
Last year, Kanzius began raising money for his research with the help of his neighbors. High school students held fundraisers, foundations offered grants, and children sold lemonade. Donations soon reached more than $1 million. This May, Erie officials gave Kanzius a key to the city and declared an official John Kanzius Day. A former Erie mayor announced a goal of raising $3 million to fund research.
But the accolades meant little if the wider medical community did not recognize the research. It had to be reviewed by a panel of medical experts and published in a scientific journal.
In June, scientists submitted manuscripts based on the findings to journals. Three months later, Curley called Kanzius with news: The manuscripts, with Kanzius listed as a co-author, would be published in December in Cancer, an oncology medical journal. The results appeared online last week.
Kanzius hung up and yelled the news to Marianne, who was watching television downstairs. She screamed.
At 63, Kanzius is still receiving treatment for his cancer, which has recurred. He knows the process he developed may not be ready in time to save his life, but the project was never about him. "I want to see the treatment work," he said. "That would be my thanks."
For Marianne, the journey led her to question her faith in God, only to have it reaffirmed.
She is hopeful the invention will help future generations, but she lives in terror, staying up at night to make sure Kanzius is still breathing. She cannot imagine waking up without her husband beside her.
"I'm selfish," she said. "If something can help him, I would like this to help him.
"Yes, I hope."
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erika.hayasaki@latimes.com