This time, it's gentle-voiced painting teacher Bob Ross, whose catchphrases get the remix treatment.
Think "Happy little trees" and "happy little clouds," all set to music.
Once again, PBS turned to Symphony of Science's John D. Boswell, also known as Musicsheep, to create the clip.
While the theme of the Mister Rogers remix was imagination, the Bob Ross remix is more about creativity and how making art can lift your spirits.
"I believe, I believe, that everyday is a good a day when you paint," he says/sings.
And later: "This is your world, you are the creator. Find freedom on this canvas."
Midway through the song, you may notice some unexpected sexual overtones. We did. But just when you think it's taking an interesting turn, it all sort of rights itself again.
Ross' show, "The Joy of Painting," aired from 1983 to 1994. He died of cancer in 1995, at the age of 52. In a 1991 interview with the New York Times, he said he had painted an estimated 30,000 works over the course of his life.